The messenger-like properties of the poly(A)+ RNA in mammalian mitochondria

This is the first demonstration that the poly(A)-containing RNA from mammalian mitochondria behaves like messenger RNA in mitochondrial specific protein synthesis. The mitochondrial RNA in HeLa cells is selectively labeled in the presence of camptothecin, the mitochondria isolated, lysed with weak detergents, and the mitochondrial contents sedimented in sucrose gradients. Much of the poly(A)-containing RNA sediments rapidly with polyribosome-like structures. Most important, the association of the poly(A)+ RNA with heavy structures is disrupted quantitatively by puromycin, which should be selective for RNA engaged in protein synthesis.

Abstract. This is the first demonstration that the poly(A)-containing RNA from mammalian mitochondria behaves like messenger RNA in mitochondrial specific  ...

อ่านต่อ